Levels of total proteins and electrophoresis of proteins from seminal plasma of Mantiqueira bulls

Authors

  • Terezinha Aparecida Martins Gomes de Castro Instituto de Zootecnia, Divisão Técnica Básica e Auxiliar, Seção de Reprodução e Inseminação Artificial, Nova Odessa, SP
  • Cndido Eduardo Paiva Machado Instituto de Zootecnia, Divisão Técnica Básica e Auxiliar, Seção de Reprodução e Inseminação Artificial, Nova Odessa, SP
  • Nancy Rute Liepin Instituto de Zootecnia, Divisão Técnica Básica e Auxiliar, Seção de Reprodução e Inseminação Artificial, Nova Odessa, SP

Abstract

The purpose of this paper was the determination of the total protein levels by chemical and electrophoretic methods from seminal plasma of brasilian Mantiqueira bulls. Thus, the levels of total protein were measured in hundred and sixty samples from eight bulls by the Bíuret method and the average obtained was 4,84 ± 0.058 g% of total protein. Disc electrophoresis in polyacrylamide colum was performed in Trisglycine buffer pH 8.5 and the results show that bovine seminal plasma contains 8 a 11 proteic fractions. The results show that methods employed may be recommended as an aid to the diagnostics in bulls used in artificial insemination.

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Published

2014-01-29

Issue

Section

ANIMAL REPRODUCTION

How to Cite

Levels of total proteins and electrophoresis of proteins from seminal plasma of Mantiqueira bulls. (2014). Bulletin of Animal Husbandry, 43(1), 37-44. http://35.198.24.243/index.php/bia/article/view/605

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